if you want to validate the data inside the column then the validation will apply to all the column,
else do the following,
use the yourdatagrid_MouseDown event, and write the following inside:
Dim hti As DataGrid.HitTestInfo = yourdatagrid.HitTest(e.X, e.Y)
If hti.Row <= yourdatagrid.DataSource.rows.count - 1 Then
If hti.Row = the_index_of_the_row_you_want Then
If hti.Type = Windows.Forms.DataGrid.HitTestType.Cell Then
If hti.Column = your_column Then
msbBox("You can't access");
End If
End If
End If
End If
Mohsen